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Methicillin-Resistant
Staphylococcus aureus
(MRSA) Screen
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The page below is a sample from the LabCE course
Case Studies in Clinical Microbiology
. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.
Learn more about Case Studies in Clinical Microbiology (online CE course)
Methicillin-Resistant
Staphylococcus aureus
(MRSA) Screen
Perhaps the most efficient means for detecting methicillin-resistant staphylococci in clinical laboratories is the use of the agar dilution screening test.
Illustrated in the image is a Mueller-Hinton agar plate containing 6 ug/mL of oxacillin, previously inoculated with a strain of
Staphylococcus aureus
. Oxacillin is used as a marker for methicillin resistance because it is more stable in the agar medium. Growth on this screening medium is presumptive for methicillin resistance.
Thus, in the presence of growth, as shown here, a follow-up minimum inhibitory concentration (MIC) test must be performed to determine the exact level of resistance.
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