Below are the results of a mini-panel of red cells specifically chosen to exclude other clinically significant antibodies in the presence of anti-D.
Besides an autocontrol, a positive control (Ror) was included to confirm that the mother's plasma containing the probable anti-D was reactive at the time of testing.
Recall that the results of the initial antibody screen showed that the possible (unexcluded) antibodies were anti-
D, C, E, K, Fyb, Jka, N, and s (with anti-N less likely as a cause of HDFN).
Antibodies excluded by screen cell #3 included anti-c, e, k, Fya , Jkb, M, S, P1, Lea, and Leb.
Before proceeding to the next page, assess whether the unexcluded antibodies from the initial antibody screen have been excluded by the mini-panel below using the guidelines in the antibody exclusion protocol shown to the right.
Cell | D | C | c | E | e | K | k | Fya | Fyb | Jka | Jkb | Lea | Leb | M | N | S | s | Lua | Lub | Cell | Gel IAT |
1 | 0 | 0 | + | 0 | + | + | + | + | 0 | + | 0 | + | 0 | 0 | + | + | 0 | + | 0 | 1 | 0 |
2 | 0 | 0 | + | 0 | + | 0 | + | 0 | + | + | 0 | + | + | 0 | + | 0 | + | 0 | 0 | 2 | 0 |
3 | 0 | + | 0 | 0 | + | 0 | + | + | + | 0 | + | + | + | + | 0 | + | 0 | 0 | 0 | 3 | 0 |
4 | 0 | + | + | 0 | + | + | + | 0 | 0 | + | + | 0 | 0 | + | 0 | + | + | 0 | + | 4 | 0 |
5 | 0 | 0 | + | + | 0 | 0 | + | 0 | + | 0 | + | + | 0 | + | + | 0 | + | 0 | 0 | 5 | 0 |
6 | 0 | 0 | + | + | + | 0 | + | + | + | + | + | 0 | + | + | + | + | + | 0 | + | 6 | 0 |
7 | + | 0 | + | + | + | + | + | + | + | + | + | 0 | + | + | 0 | + | + | 0 | + | 7 | 2+ |
Auto |
| | | | | | | | | | | | | | | | | | | Auto | 0 |
All panel cells are negative for low-frequency antigens and positive for high-frequency antigens unless noted otherwise. All cells are also negative for Cw, Kpa, Jsa, and Lua.