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Which of the following statements is FALSE when describing indirect immunofluorescence assays (IFAs)?
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Autoimmune Diseases and Antinuclear Antibody Testing: Methods and Staining Patterns (retired 3/30/2020)
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Which of the following statements is FALSE when describing indirect immunofluorescence assays (IFAs)?
Please select the single best answer
Fluorescein isothiocyanate (FITC) is employed as the fluorescent marker on the secondary antibody, which produces a bound complex that will fluoresce at a specific wavelength of light which can be seen under the microscope.
HEp-2 cells are currently one of the most common substrates for ANA detection by IFA.
If the sample is negative for ANA, the nucleus will show a distinct, clearly discernible pattern of nuclear fluorescence.
Although positive titers of 1:160 or higher are strongly associated with autoimmune disorders, they are also found in 5% of healthy individuals.
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