References

This version of the course is no longer available.
Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course . Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about (online CE course)
References

Applied Biosystems. Factors Influencing Multiplex Real-Time PCR. Available at: http://www3.appliedbiosystems.com/cms/groups/mcb_marketing/documents/generaldocuments/cms_076529.pdf. Accessed October 3, 2018.
Berkowitz FE, Jerris RC. Practical Medical Microbiology for Clinicians. Hoboken, NJ: John Wiley and Sons; 2016.
Burd, EM. Validation of Laboratory-Developed Molecular Assays for Infectious Diseases. Clin Microbiol Rev. 2010;23:550-576.
Clinical and Laboratory Standards Institute (CLSI). Validation and Verification of Multiplex Nucleic Acid Assays; Approved Guideline. CLSI document MM-17 2nd ed. Wayne, PA: CLSI; 2018.
Edward MC, Gibbs RA. Multiplex PCR: advantages, development, and applications. Genome Res. 1994;3:S65-S75.
Elnifero EM, Ashshi AM, Cooper RJ, Klapper PE. Multiplex PCR: Optimization and Application in Diagnostic Virology. Clin Microbiol Rev. 2000;13:559-570.
Laboratory for Environmental Pathogens Research, Department of Environmental Sciences, University of Toledo. Polymerase Chain Reaction. 2004. Available at: http://www.eeescience.utoledo.edu/Faculty/Sigler/Von_Sigler/LEPR_Protocols_files/PCR.pdf. Accessed October 3, 2018.
Markoulatus P, Siafakas N, Moncay M. Multiplex Polymerase Chain Reaction: A Practical Approach. J Clin Lab Anal. 2002;16:47-51.
Mullis KB. The Unusual Origin of the Polymerase Chain Reaction. Scientific American.1990;262: 56-61.
OIE Validation Guidelines 2014. Validation Guideline 3.6.3.: Development and Optimisation of Nucleic Acid Detection Assays. Available at: http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/current/GUIDELINE_3.6.3_NAD_ASSAYS.pdf. Accessed October 3, 2018.
Palavacino E. One Sample, Multiple Results: The Use of Multiplex PCR for Diagnosis of Infectious Syndromes. Clinical Laboratory News. Washington, DC: AACC; 2015. Available at: https://AACC.org/publications/cln/articles/2015/april/one-sample-multiple-results. Accessed October 3, 2018.
PCR Primer Design Guidelines. Premier Biosoft website. Available at: http://premierbiosoft.com/tech_notes/PCR_Primer_Design.html. Accessed October 3, 2018.
Perkel JM. Overcoming the Challenges of Multiplex PCR. Biocompare website. 2012. Available at: http://www.biocompare.com/Editorial-Articles/117895-Multiplex-PCR/. Accessed October 3, 2018.
Rogers BB, Shankar P, Jerris RC, Kotzbauer D, Anderson EJ, et al. Impact of a rapid respiratory panel test on patient outcomes. Arch Pathol Lab Med. 2015 May;139(5):636-641. Available at: http://www.ncbi.nlm.nih.gov/pubmed/25152311. Accessed October 3, 2018.