Group A Strep A Disk/SXT

This version of the course is no longer available.
Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course Case Studies in Clinical Microbiology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Case Studies in Clinical Microbiology (online CE course)
Group A Strep A Disk/SXT

In follow up to the previous question, the upper image again illustrates the colonies recovered from the blood culture bottle. The colonies are small, transluscent, gray-yellow, and surrounded by a wide zone of beta hemolysis. The size of the colonies compared to the zones of hemolysis suggests a group A Streptococcus.
The susceptibility to bacitracin (zone of inhibition around the "A" disk in the lower image) is virtually diagnostic of a group A Streptococcus.
The absence of a zone of inhibition around the sulfamethoxazole/trimethoprim (SXT) disk indicates resitance to sulfamethoxazole/trimethoprim. SXT resistance is also shared by group B streptococci, which are, however, resistant to bacitracin. The resistance to SXT is used for the primary recovery of groups A and B streptococci from specimens with mixed culture. Their resistance allows them to selectively grow out from contaminating bacteria that are inhibited by this antibiotic.