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Phenotypic Confirmation by Broth Microdilution and Reporting
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Case Studies in Clinical Microbiology
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Phenotypic Confirmation by Broth Microdilution and Reporting
Phenotypic confirmation by broth microdilution is performed using either of the previously mentioned antimicrobial agents and testing in combination with clavulanic acid. A ≥ 3 twofold concentration decrease in an MIC between the agent tested alone, versus testing in combination with clavulanate is taken to be phenotypic confirmation of ESBL production.
The same reporting should be followed as for the phenotypic confirmation by disc diffusion
ONLY IF
the new CLSI cephalosporin and aztreonam interpretive criteria have
NOT
been implemented.
If using the current criteria, test interpretations do not need to be changed from susceptible to resistant.
X
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