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Phenotypic Confirmation by Disk Diffusion and Reporting
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Case Studies in Clinical Microbiology
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Phenotypic Confirmation by Disk Diffusion and Reporting
Phenotypic confirmation by disc diffusion is performed using either of the previously mentioned antimicrobial agents and testing in combination with clavulanic acid. A ≥5 mm increase between the zone diameters of either agent tested alone, versus testing in combination with clavulanate is taken to be phenotypic confirmation of ESBL production.
When reporting confirmed ESBL-producing strains, the interpretation should be reported as
resistant
for all penicillin's, cephalosporin's, and aztreonam
ONLY IF
the current CLSI cephalosporin and aztreonam interpretive criteria has
NOT
been implemented.
If using the current criteria, test interpretations do not need to be changed from susceptible to resistant.
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