Solubility Test

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Solubility Test

Most hemoglobins are soluble in a high-molarity phosphate buffer; hemoglobin S is not. The buffer is made of of dibasic and monobasic potassium phophates, Saponin and dithionate. Kits are available, which consist of this reagent, pipets and a reading rack. A 1:100 dilution of blood into buffer is made, incubated for 5 minutes, and turbidity is observed against a white background with black lines.

A positive result (A below) is indicated by a turbid solution.

A negative result (B below) is obtained when lines are visible through the solution.

The solubility test should only be used as a screening test as it is not reliable for diagnosing sickle cell disease.