Various methods have been employed for detection of C. difficile disease: cultivation of the organism, cell cytotoxin neutralization assays, and enzyme immunoassays have all been among the staples of diagnostic approaches. Which statements are accurate characterizations of these assays?

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Various methods have been employed for detection of C. difficile disease: cultivation of the organism, cell cytotoxin neutralization assays, and enzyme immunoassays have all been among the staples of diagnostic approaches. Which statements are accurate characterizations of these assays?

More than one answer is correct. Please select all correct answers

	Cultivation on CCFA agar is relatively easy to perform and is uniformly diagnostic.
	Cell culture based cytotoxin neutralization assays provide definitive detection of toxin-producing C. difficile strains.
	Enzyme immunoassays detecting Toxin A provide adequate detection and more efficient methodologies compared to culture or cell culture based assays.
	Enzyme immunoassays detecting both Toxin A and Toxin B allow the detection of more strains of C. difficile and are therefore preferred over assays that detect only Toxin A.