Failure to determine accurate decalcification end-point results in the loss of basophilia which subsequently leads to an increase in eosin staining, with a lack of nuclear staining in the H&E stain.

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Failure to determine accurate decalcification end-point results in the loss of basophilia which subsequently leads to an increase in eosin staining, with a lack of nuclear staining in the H&E stain.

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