Sample type required: Deparaffinized and rehydrated tissue section (6 - 8μm) on positively (+) charged slides
Preferred fixative: 10% neutral buffered formalin (NBF)
Control: Normal spinal cord
0.5% cresyl echt violet | 2 minutes | |
Distilled water | 3 changes | |
70% alcohol | Differentiate until background is colorless | Check microscopically for endpoint |
Post staining procedure: Tissue section should be dehydrated through graded alcohols beginning with 95% immediately following the differentiation step. Follow with two changes of xylene and then coverslip.
Expected Results:
- Nissl substance - Dark blue to purple