Example of Clinically Significant Immune Antibody

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Example of Clinically Significant Immune Antibody

The panel below shows reactions, noted in deep blue, in the AHG phase only. The pattern reactivity of the sample matches the pattern displayed by C on the panel. Anti-C is a clinically significant antibody that can cause both hemolytic disease of the fetus and newborn (HDFN) and hemolytic transfusion reaction (HTR).
Rh-HrKellDuffyKiddLewisMNSsPLutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+03+NT
2++00++++0+0+++0+0+0++++0+03+NT
3+0++000+0+0+0+0+0++00++0+002+
4+0+0+00+0+0+00+00++++0+0+002+
50++0+00+0+0++++++00+0+00+02+NT
600+++0++0+0+0+++0+++++00+002+
700+0+00+0+0++00++0+00++0+002+
800+0+00+0+0+++0+0+++++00+002+
900+0+00+0+0++++00+0++0+0+002+
10+0+0+0++0+0+0+0+0+0++0+0+002+
AC 002+
IS = immediate spin; AHG = antihuman globulin; CC = check cells; NT = not tested; AC = autocontrol