Assessing FMH and RhIG Dosage

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The page below is a sample from the LabCE course Rh-Negative Mother with Anti-D at Delivery: A Case Study. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Assessing FMH and RhIG Dosage

The remaining issue in this case is to determine if one vial of RhIG is sufficient or if there has been an FMH >30 mL of whole blood, requiring more than one vial of RhIG (300 µg).
Recall that the incidence of FMH greater than 30 mL at delivery is rare and estimated to be about 1 in 400 deliveries (~0.3%).
The laboratory used the rosette test to screen for FMH and it was negative. Accordingly, quantitation using the KB test or flow cytometry was not needed.
RhIG Dosage:
Based on the negative rosette test, the mother was injected with one vial of RhIG (300 µg). The mother was later discharged along with the healthy infant.