The table below delineates some of the more commonly seen false-negative reactions with Rh typing reagents and provides insight into the causes and corrective actions that may be undertaken when these problems are presented.
Failure to follow manufacturer's instructions precisely | Review direction circular; repeat test |
Resuspension too vigorous | Resuspend all tubes gently during repeat testing |
Immunoglobulin-coated cells (in vivo) | Use saline-active typing reagent; repeat test |
Incorrect reagent selected | Read vial carefully; repeat test |
Reagent deterioration | Open new vial, perform appropriate QC; repeat test |
Modified from Harmening, D: Table 7-10, Modern blood banking and transfusion practices, 6thed, FA Davis, Philadelphia, PA, 2012, p 162.
Fung, MK, Eder, AF, Spitalnik, SL, Westhoff, CM (eds): Technical Manual, 19th ed. AABB, Bethesda, MD, 2017, p 313.