Interpretation of ABO Group

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The page below is a sample from the LabCE course Introduction to the ABO Blood Group System. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Interpretation of ABO Group

We can use the forward type with the reverse type to interpret the ABO group. The expected reactions are listed in Table 7.
Table 7. Interpretation of ABO Group.
Red Cells Tested With Known Antisera
Serum Tested With Known Red Cells
Interpretation of ABO Group
Anti-A
Anti-B
Anti-A,B
A1 Cells
B Cells
3-4+
0
3-4+
0
3-4+
A
0
3-4+
3-4+
3-4+
0
B
0
0
0
3-4+
3-4+
O
3-4+
3-4+
3-4+
0
0
AB
+ = agglutination (graded 1+ to 4+)
0 = no agglutination or hemolysis