Testing Patient Serum With Known Reagent Red Cells (Reverse Grouping)

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The page below is a sample from the LabCE course Introduction to the ABO Blood Group System. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Testing Patient Serum With Known Reagent Red Cells (Reverse Grouping)

Table 6. Testing Patient Serum With Known Reagent Red Cells (Reverse Grouping).
Patient Serum Tested With Known Reagent Red Cells
Antibodies Present in Serum
A1 Cells
B Cells
0
3-4+
Anti-B
3-4+
0
Anti-A
3-4+
3-4+
Anti-A and Anti-B
0
0
No ABO antibodies present
+ = agglutination (graded 1+ to 4+)
0 = no agglutination or hemolysis