The staining protocol for H&E follows a basic protocol:
- Dewaxing
- Dehydration
- Hematoxylin
- Differentiation
- Bluing
- Eosin
- Dehydration
- Clearing
- Coverslipping
The format is easily reproduced and the reagent resilient enough to allow for large numbers of slides to be stained consistently before reagents need to be changed.