Level of instruction: Intermediate
Intended Audience: Clinical laboratory histotechnologists and technicians,and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.
Author information: Anita Buchiane, HT(ASCP) QHIC, is the Lead Histology Technician at Brattleboro Memorial Hospital in Brattleboro, Vermont. Prior to this position, she worked as a Neuropathology/Special Procedures Technician at Baystate Medical Center in Springfield, Massachusetts. Ms. Buchiane is a graduate of Hartford Hospital School of Allied Health in Connecticut, where she earned her Certificate in Histotechnology, and Greenfield Community College, Greenfield, Massachusetts, where she earned an associates degree in Liberal Arts with a math and science concentration. She is a member of the National Society for Histotechnology and the VT/NH Society for Histotechnology. She was the recipient of the 2007 Lee G. Luna Foreign Travel Scholarship awarded by the National Society for Histotechnology and the 2010 Region 1 Scholarship awarded by the VT/NH Society for Histotechnology.
Reviewer information: James R. Pepoon, BS, HT(ASCP) QIHC is the Administrative Director of Clinical Operations and Planning at Brigham and Women's Hospital in Boston.
Course Description: This course will explore the history and application of hematoxylin and eosin (H&E) stains used in the clinical histology laboratory. Basic H&E staining mechanisms and the classification of biological stains will be discussed. The material presented details the chemistry, diagnostic application, and problem solving strategies for H&E stains.