Level of instruction: Intermediate
Intended audience: Medical laboratory scientists, medical technologists, and technicians, This course is also appropriate for clinical laboratory science students and pathology residents.
Author information: Ryanne Wolfe holds an MS in biotechnology and an MBA from the University of Maryland. Ryanne has worked in the health care field for eight years, most recently in the laboratory at St. Christopher’s Hospital for Children in Philadelphia, Pennsylvania.
Reviewer information: Dana L. Van Laeys, MA, CLSpMB, MT, CLS is the Education Coordinator for Molecular Diagnostics and Immunology in the Clinical Laboratory Science Program at Saint Luke’s Hospital in Kansas City, Missouri, a position that she has held since 1998. She has 17 years of experience in molecular diagnostics and flow cytometry. She has been a presenter at ASM, CLEC, and AABB conferences. Ms. Van Laeys holds a Masters degree in Adult Education and Distance Learning.
Course information: This course provides a brief overview of DNA and RNA structure and the principles of polymerase chain reaction. It then proceeds to discuss the principles and procedures for real-time PCR, highlighting the advantages over traditional PCR methods and the applications in the clinical setting.