HIV: Structure, Replication, and Detection (Online Course)

(based on 672 customer ratings)

Author: Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB)
Reviewer: Barbara Cebulski, MS, MLS(ASCP)

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Continuing Education Credits

  • P.A.C.E.® Contact Hours: 1.5 hour(s)
  • Florida Board of Clinical Laboratory Science CE - General (Serology/Immunology): 1.5 hour(s)

Objectives

  • Explain the basic structure of HIV.
  • Discuss the process by which HIV replicates.
  • Discuss commonly used laboratory methods for detection of HIV infection.
  • Compare the advantages and disadvantages of commonly used laboratory test methods for detection of HIV infection.

Customer Ratings

(based on 672 customer ratings)

Course Outline

  • Structure of the Human Immunodeficiency Virus
      • Basic Structural Components
      • HIV Envelope
      • HIV Capsid
      • Viral proteins are identified as either glycoprotein (gp) or protein (p). What is indicated by the number that follows the "gp" or "p" designation, fo...
  • Replication
      • Proteins Involved in Adsorption
      • Adsorption
      • Adsorption, continued
      • Penetration and Infection
      • DNA Conversion from RNA
      • HIV is a Retrovirus
      • Infection of the Host Cell
      • Infection of the Host Cell, continued
      • Spread of Infection
      • Spread of Infection, continued
      • Spread of Infection, continued
      • Destruction of the Host Cell
      • What is the protein that coats the genome in the core of the HIV-1 virion?
      • The enzyme found in the core of the HIV virion that is utilized to convert viral RNA into DNA is called reverse transcriptase.
  • HIV Virus Classifications
      • Mutations
      • Most HIV-1 infections are classified as which one of the following groups?
  • Progression of HIV Infection
      • Stages of HIV Infection
      • Which stage of HIV infection is characterized by a decline in the CD4 T-lymphocyte count to less than 200 cells/µL?
  • Laboratory Methods to Detect and Monitor HIV Infection
      • HIV Test Methods: Introduction
      • Anti-HIV Immunoassays
      • HIV Immunoassay Methods
      • Rapid Anti-HIV Assays Using Alternate Specimen Types
      • Western Blot
      • Nucleic Acid Testing (NAT)
      • Reverse Transcriptase PCR (RT-PCR)
      • HIV Detection Methods: Comparison Table
      • The Centers for Disease Control and Prevention (CDC) Recommended Algorithm for HIV Testing
      • Which of the following HIV tests would be the BEST CHOICE to detect HIV infection in a high-risk seronegative individual?
  • References
      • References

Additional Information

Level of instruction: Intermediate
Intended audience: Medical laboratory technologists and technicians and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
Author information: Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB) received his MSPH from the University of Kentucky in Lexington, his PhD in medical parasitology and mycology from the University of North Carolina in Chapel Hill, and his law degree with a concentration in health care law from Concord Law School, Purdue University. He also did a Fellowship in Tropical Medicine at Louisiana State University School of Medicine. He is the author of a textbook in phlebotomy, a number of scientific articles, plus internet training programs. He is licensed as a laboratory director in the States of Georgia and Florida.
Reviewer: Barbara Cebulski, MS, MLS(ASCP) has over 40 years of experience in the medical laboratory profession as a technologist, section supervisor, and laboratory manager. She was an Inspection and Technical Specialist for nine years with the College of American Pathologists in the Laboratory Accreditation Program and, until her retirement in 2015, was Program Director for MediaLab, Inc. Barbara holds a Masters in Instructional Technology from Georgia State University.




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