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Histology Special Stains: Connective Tissue (Online Course)

(based on 226 customer ratings)

Author: Tanisha N. Neely, HT(ASCP)
Reviewer: Diana Harrington, BS, HT(ASCP)

This course is the second in a series of courses that will explore special stains used in the clinical histology laboratory. Basic connective tissue staining mechanisms and classifying connective tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate connective tissue in samples.

This course is part of MediaLab's Histology Compliance & CE package, which includes over 20 hours of continuing education for histologists. View histology courses ».

This course is not included as part of the Compliance & CE package.

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Continuing Education Credits

Objectives

  • Understand basic tissue staining methods used in the clinical histology laboratory.
  • Explain factors that affect dye binding and utilize to troubleshoot staining issues.
  • Classify connective tissue elements and identify which dyes will bind to these elements.
  • Demonstrate safe microwave use when necessary to perform special staining procedures.

Customer Ratings

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Course Outline

Click on a link in the outline to view a sample page from this course.

  • Introduction
      • Tissue Staining in the Clinical Histology Laboratory
      • The Biological Stain Commission (BSC)
      • Biological dyes can be grouped into which of the following categories?
      • Natural dyes greatly outnumber artificial dyes.
  • Basic Staining Mechanisms
      • What Makes a Dye?
      • Factors That Affect Dye Binding
      • Acids, Bases, pH, and Buffers
      • Basophilic and Acidophilic Staining
      • Oxidation and Reduction
      • For tissue staining to occur, chromogens in a dye solution must bind to the desired elements through what process?
      • What would a solution with a pH of 6.9 be considered?
  • Connective Tissue Classification and Functions
      • What is Connective Tissue?
      • Connective Tissue Components - A Visual Aid
      • Classifying Connective Tissue
      • Functions of Connective Tissue
      • Where are reticular fibers found?
      • Cartilage is composed of collagen, elastin, and reticulin fibers as well as adipose and mast cells.
  • Special Staining Methods Used to Demonstrate Connective Tissue
      • Gordon and Sweet's Silver Staining - Chemistry
      • Gordon and Sweet's Silver Staining - Diagnostic Applications
      • Gordon and Sweet's Silver Staining - Staining Protocol
      • Verhoeff-Van Gieson (VVG) Staining - Chemistry
      • Verhoeff-Van Gieson (VVG) Staining - Diagnostic Applications
      • Verhoeff-Van Gieson (VVG) Staining - Staining Protocol
      • Toluidine Blue Staining - Chemistry
      • Toluidine Blue Staining - Diagnostic Applications
      • Toluidine Blue Staining - Staining Protocol
      • Masson's Trichrome Staining - Chemistry
      • Masson's Trichrome Staining - Diagnostic Applications
      • Masson's Trichrome Staining - Staining Protocol
      • Phosphotungstic Acid-Hematoxylin (PTAH) Staining - Chemistry
      • Phosphotungstic Acid-Hematoxylin (PTAH) Staining - Diagnostic Applications
      • Phosphotungstic Acid-Hematoxylin (PTAH) Staining - Staining Protocol
      • Which of the following staining procedures can be used to demonstrate elastic fibers in connective tissues?
      • Excess silver pigments are removed in the Gordon and Sweet's silver staining procedure using which of the following?
  • Using the Microwave for Special Staining Procedures
      • Microwaves in the Histopathology Laboratory
      • Tips for using the Microwave for Special Staining Procedures
      • Safety Precautions for Microwave Usage
      • Containers used in a microwave oven should be tightly sealed to prevent liquids from bubbling over.
      • What should users do to prevent high dose exposure to microwaves? (Choose the BEST response.)
  • References
      • References

Additional Information

Level of instruction: Intermediate
 
Intended audience:  Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.  
 
Author information: Tanisha N. Neely, HT(ASCP) is a freelance writer with a work history in histology, microbiology, and pharmaceutical research. Ms. Neely attended Indiana University where she earned a bachelors degree in arts & humanities from the School of General Studies and a Certificate in Histotechnology from the School of Medicine. She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.
 
Reviewer information: Diana Harrington, BS, HT(ASCP) is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
 
Course description: This course is the second in a series of courses that will explore special stains used in the clinical histology laboratory. Basic connective tissue staining mechanisms and classifying connective tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate connective tissue in samples. 





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