CAP Pro Course - Microbiology - The Microbiology of Wounds

Continuing Education Credits

• P.A.C.E.® Contact Hours: 1 hour(s)
• Florida Board of Clinical Laboratory Science CE Credit Hours -
  General (Microbiology/Mycology/Parasitology): 1 hour(s)

Objectives

• Identify the optimal specimen types to be submitted for assessment of various wound infections.
• Define appropriate methods for wound culture collection and describe how the specimen type may impact the clinical relevance of the results.
• Outline appropriate specimen processing and plating for wound culture specimens.
• Be able to list the common pathogens isolated from wound cultures, describe their Gram stain result and appearance on routine culture media.
• Describe different strategies for the evaluation and work up of wound cultures that provide effective and appropriate guidance for patient therapy.

Course Outline

• Identify the optimal specimen types to be submitted for assessment of various wound infections.
• Ideal Specimens Used to Diagnose Wound Infections
• Types of Wound Specimens Submitted for Microbiological Processing
• Descriptive Terms for Dermal and Epidermal Lesions and Infections
• What type of infection is a wound involving the hair follicle that is described as a papule pierced by hair and surrounded by redness, without pus?
• In general, what is the preferred specimen for a wound culture workup?
• A patient presents to their physician with a wound that the physician describes as a crater-like defect with loss of the epidermis and dermis on the g...
• Which of the following statements is not true about collecting a wound specimen for culture?
• Define appropriate methods for wound culture collection and describe how the specimen type may impact the clinical relevance of the results.
• Essential Aspects of Wound Culture Collection
• Collection Methods for Various Types of Wounds
• Necrotizing Lesions: Definitions and Specimen Collection
• Clinical Relevance of Results of Various Wound Cultures: Do's and Don'ts
• Which of the following describes the preferred collection method when collecting a superficial wound specimen?
• This infection is painful and involves subcutaneous tissues. It may be due to an underlying decubitus ulcer. If suspected, it is an emergency surgical...
• This type of necrotizing lesion is an indolent process involving the muscle and fascia; it is usually associated with trauma or surgical procedures. B...
• A physician has a patient presenting with an open abscess on the arm of unknown origin. The patient denies any animal or human bites and has not had a...
• A patient presents with necrotic tissue in his toe due to inadequate blood supply. During surgical debridement and amputation of the affected toe, the...
• Outline appropriate specimen processing and plating for wound culture specimens.
• Culture Requests Based on Wound Type
• Processing and Media Selection: General Guidelines
• Processing and Media Selection: Additional Information
• Processing and Media Selection: Non-routine Requests
• Specimen Processing: Tissue Samples
• Which of the following wound types should not be included in an anaerobic culture?
• Which of the following would indicate the necessity for including a broth medium in the culture preparation?
• When should a chocolate agar be added to the minimum plating protocol?
• A professional landscaper presents to his physician complaining of a wound on his knee that he suspects occurred after kneeling on a rose bush.The phy...
• A 51-year-old coastal fisherman presented to his physician with cellulitis on his right arm. The physician cultured the site and sent it to microbiolo...
• Be able to list the common pathogens isolated from wound cultures, describe their Gram stain result and appearance on routine culture media.
• Common Organisms Causing Wound Infections
• Common Aerobic Organisms Isolated From Wound Cultures
• Common Anaerobic Organisms Isolated From Wound Cultures
• Expected Gram Stains in Common Wound Infections
• Expected Gram Stains in Common Wound Infections, continued
• The image to the right depicts the Gram stain observed from a small white colony found only on the anaerobic culture. What is the most likely identifi...
• A 28-year-old woman presented to her physician for a follicular wound on her foot that has progressively deteriorated over the last week and a half. T...
• When reviewing the Gram stain from a foot wound on a 65-year-old female patient, you notice small gram-negative rods that exhibit bipolar staining. Af...
• Understand the different strategies for the evaluation and work up of wound cultures that provide effective and appropriate guidance for patient therapy.
• Overall Principles of Working up Wound/Abscess Cultures
• Determining the Extent of Workup: Q Score
• Determining the Extent of Workup Based on Number of Potential Pathogens in Culture
• Which of the following principles concerning the workup of wound/abscess cultures is true?
• This strategy is used to bring standardization to wound culture workups. It is based on the number of polymorphonuclear (PMN) cells compared to the nu...
• While working up a wound culture from an arm abscess, there are enteric gram-negative rods and a moderate amount of coagulase-negative staphylococci a...
• In your microbiology laboratory, you utilize the Q score to determine the extent of the work up performed on wound cultures. When reviewing an abscess...
• References
• References

Additional Information