Bone Marrow Aspiration: Normal Hematopoiesis and Basic Interpretive Procedures (Online Course)

(based on 154 customer ratings)

Author: Marybeth Helfrich, MT(ASCP)
Reviewer: Patricia Boyer, MSHS, MLS (ASCP)CM

This course provides a thorough review of cellular elements encountered in normal bone marrow. It also provides guidelines for preparation of smears, including an instructional video, and evaluation and reporting of bone marrows.

See all available courses »

Continuing Education Credits

Objectives

  • Describe bone marrow sample collection, smear preparation and routine staining techniques.
  • Recognize and identify myeloid and erythroid precursors found in normal bone marrow aspirates.
  • Compare lymphocytes, hematogones and plasma cells.
  • Compare and contrast monocytes, monocyte precursors and macrophages.
  • Describe benign background cell types found in normal bone marrow.
  • Outline and explain standard procedures for reporting bone marrow differentials.

Customer Ratings

(based on 154 customer ratings)

Course Outline

  • Bone Marrow Aspirates
      • Introduction to Bone Marrow Aspirates and Biopsies
      • Bone Marrow Biopsy
      • Bone Marrow Aspirate and Biopsy Collection
      • Clinical Laboratory's Role: Bone Marrow Aspirates and Biopsies
      • In which of the following situations would a bone marrow aspirate and biopsy possibly be used as an aid for diagnosis?(Select all that apply)
    • Sample Collection
      • Bone Marrow Collection: Patient Bedside
      • Bone Marrow Delivery
      • The role of the laboratory technologist in processing bone marrow aspirates can vary depending on laboratory and clinician protocols. Which of the fol...
    • Smear techniques
      • Bone marrow Smear Preparation
      • Bone marrow Smear Preparation: Selecting Fragments
      • Long Slide Preparation Techniques: Wedge Smear
      • Long Slide Preparation Techniques: T-prep
      • Long Slide Preparation Techniques: Pull Prep
      • Coverslip Smear Preparation Technique
      • Biopsy Touch Preparation Technique
      • Bone Marrow Smear Techniques Video
      • Which of the following smear techniques can be utilized when processing bone marrow aspirate samples? (Choose all that apply)
    • Routine Staining
      • Manual Staining of Bone Marrow Preparations
      • Manual Staining of Bone Marrow Preparations: Wright's and Wright-Giemsa Stain
      • Automated Stainers
      • Iron Staining
      • The same staining protocol can be used for bone marrow smears as is used for peripheral blood smears.
  • Normal Bone Marrow Morphology
      • Bone marrow Differentials
      • Rules for Bone Marrow Differentials
      • Rules for Bone Marrow Differentials, continued
      • Rules for Bone Marrow Differentials, continued
    • Myeloid Maturation Sequence
      • Myeloblast
      • Promyelocyte
      • Myelocyte
      • Myelocytes: Eosinophils and Basophils
      • Metamyelocyte
      • Band Neutrophil
      • Segmented Neutrophil
      • Place the following stages of neutrophilic granulocyte cellular development in the correct order of maturation, beginning with the earliest form:
    • Erythroid Maturation Sequence
      • Erythrocytic Cells: Introduction
      • Pronormoblast (Proerythroblast)
      • Basophilic Normoblast
      • Polychromatophilic Normoblast
      • Orthochromic Normoblast
      • When performing bone marrow differentials it is not necessary to distinguish the precursor forms of the erythroid sequence.
    • Monocytes / Macrophages
      • Monoblast
      • Promonocyte
      • Monocyte
      • Macrophage (Histiocyte)
      • Hemophagocytosis
    • Lymphocytes / Hematogones / Plasmacells
      • Lymphocyte
      • Hematogone
      • Plasma Cell
      • What are the cells that are indicated by the red arrows in the image on the right?
    • Megakaryocytes
      • Megakaryocyte: Immature
      • Megakaryocyte: Mature
    • Other Constituents of Normal Bone Marrow
      • Osteoclast
      • Osteoblast
      • Stromal Cells
  • Bone Marrow Evaluation and Reporting
      • Calculating and Reporting the Myeloid:Erythroid (M:E) Ratio
      • Cellularity and Additional Information
      • Responsibility of the Technologist versus Hematopathologist
  • References
      • References

Additional Information

Level of Instruction: Intermediate to Advanced

Intended Audience: Clinical laboratory technologists, technicians, and pathologists. This course is also appropriate for clinical laboratory science students and pathology residents.
Author Information: Marybeth Helfrich, MT(ASCP) is currently a Laboratory Technologist Specialist for the Hematology/Oncology Laboratory at Childrens Hospital of Philadelphia. She received her BS, MT from Temple University in Philadelphia and has nearly 40 year experience in both adult and pediatric hematology and oncology settings. She is a regular presenter for ASCLS-PA, has been a presenter for ASCP (local and national), and the Texas Teleconference network. She is responsible for hematology morphology instruction and training for medical technologists and fellows. She is also a regular developer of morphology tutorials for in-house continuing education activities.
Reviewer Information: Patricia Boyer, MSHS, MLS(ASCP)CM is the Program Director of the Zablocki VAMC School of Medical Technology and an adjunct professor at Marquette University and the University of Wisconsin-Oshkosh. She has a Master’s of Science in Health Sciences with an emphasis on professional education. She currently teaches in the areas of hematology, transfusion medicine, and hemostasis.
Course information: This course provides a thorough review of cellular elements encountered in normal bone marrow. It also provides guidelines for preparation of smears, including an instructional video, and evaluation and reporting of bone marrows.




Just one user? Visit LabCE.com for individual subscriptions.
band 2 sml


rbc quiz 1 sml


seg sml


stroma sml


erythroblast pronormoblast 2 sml with arrows


bm baso + poly sml with arrows


2jmml13 sml with arrows