Bone Marrow Aspiration: Normal Hematopoiesis and Basic Interpretive Procedures (Online Course)

(based on 285 customer ratings)

Author: Marybeth Helfrich, MT(ASCP)
Reviewer: Paul S. Fekete, MD, FCAP

This course provides a thorough review of cellular elements encountered in normal bone marrow. It also provides guidelines for preparation of smears, including an instructional video, and evaluation and reporting of bone marrows.

See all available courses »

Continuing Education Credits

Objectives

  • Describe bone marrow sample collection, smear preparation and routine staining techniques.
  • Recognize and identify myeloid and erythroid precursors found in normal bone marrow aspirates.
  • Compare lymphocytes, hematogones and plasma cells.
  • Compare and contrast monocytes, monocyte precursors and macrophages.
  • Describe benign background cell types found in normal bone marrow.
  • Outline and explain standard procedures for reporting bone marrow differentials.

Customer Ratings

(based on 285 customer ratings)

Course Outline

  • Bone Marrow Aspirates
      • Introduction to Bone Marrow Aspirates and Biopsies
      • Bone Marrow Biopsy
      • Bone Marrow Aspirate and Biopsy Collection
      • Clinical Laboratory's Role: Bone Marrow Aspirates and Biopsies
      • In which of the following situations would a bone marrow aspirate and biopsy possibly be used as an aid for diagnosis?(Select all that apply)
    • Sample Collection
      • Bone Marrow Collection: Patient Bedside
      • Bone Marrow Delivery
      • The role of the laboratory technologist in processing bone marrow aspirates can vary depending on laboratory and clinician protocols. Which of the fol...
    • Smear techniques
      • Bone marrow Smear Preparation
      • Bone marrow Smear Preparation: Selecting Fragments
      • Long Slide Preparation Techniques: Wedge Smear
      • Long Slide Preparation Techniques: T-prep
      • Long Slide Preparation Techniques: Pull Prep
      • Coverslip Smear Preparation Technique
      • Biopsy Touch Preparation Technique
      • Bone Marrow Smear Techniques Video
      • Which of the following smear techniques can be utilized when processing bone marrow aspirate samples? (Choose all that apply)
    • Routine Staining
      • Manual Staining of Bone Marrow Preparations
      • Manual Staining of Bone Marrow Preparations: Wright's and Wright-Giemsa Stain
      • Automated Stainers
      • Iron Staining
      • The same staining protocol can be used for bone marrow smears as is used for peripheral blood smears.
  • Normal Bone Marrow Morphology
      • Bone marrow Differentials
    • Myeloid Maturation Sequence
      • Myeloblast
      • Promyelocyte
      • Myelocyte
      • Myelocytes: Eosinophils and Basophils
      • Metamyelocyte
      • Band Neutrophil
      • Segmented Neutrophil
      • Place the following stages of neutrophilic granulocyte cellular development in the correct order of maturation, beginning with the earliest form:
    • Erythroid Maturation Sequence
      • Erythrocytic Cells: Introduction
      • Pronormoblast (Proerythroblast)
      • Basophilic Normoblast
      • Polychromatophilic Normoblast
      • Orthochromic Normoblast
      • When performing bone marrow differentials it is not necessary to distinguish the precursor forms of the erythroid sequence.
    • Monocytes / Macrophages
      • Monoblast
      • Promonocyte
      • Monocyte
      • Macrophage (Histiocyte)
      • Hemophagocytosis
    • Lymphocytes / Hematogones / Plasmacells
      • Lymphocyte
      • Hematogone
      • Plasma Cell
      • What are the cells that are indicated by the red arrows in the image on the right?
    • Megakaryocytes
      • Megakaryocyte: Immature
      • Megakaryocyte: Mature
    • Other Constituents of Normal Bone Marrow
      • Osteoclast
      • Osteoblast
      • Stromal Cells
  • Bone Marrow Evaluation and Reporting
      • Rules for Bone Marrow Differentials
      • Rules for Bone Marrow Differentials, continued
      • Rules for Bone Marrow Differentials, continued
      • Calculating and Reporting the Myeloid:Erythroid (M:E) Ratio
      • Cellularity and Additional Information
      • Responsibility of the Technologist versus Hematopathologist
  • References
      • References

Additional Information

Level of Instruction: Intermediate to Advanced

Intended Audience: Clinical laboratory technologists, technicians, and pathologists. This course is also appropriate for clinical laboratory science students and pathology residents.
 
Author Information: Marybeth Helfrich, MT(ASCP) is currently a Laboratory Technologist Specialist for the Hematology/Oncology Laboratory at Childrens Hospital of Philadelphia. She received her BS, MT from Temple University in Philadelphia and has nearly 40 year experience in both adult and pediatric hematology and oncology settings. She is a regular presenter for ASCLS-PA, has been a presenter for ASCP (local and national), and the Texas Teleconference network. She is responsible for hematology morphology instruction and training for medical technologists and fellows. She is also a regular developer of morphology tutorials for in-house continuing education activities.
 
Reviewer Information: Paul S. Fekete, MD, FCAP is the President and CEO of MediaLab, Inc. He received his MD from Ohio State University and completed his clinical pathology residency at Emory University in Atlanta. He has served as an Assistant Professor of Pathology at Emory and as the Director of Laboratories for Gwinnett Medical Center in Lawrenceville, GA. He is currently Assistant Professor of Pathology, at the Augusta University University of Georgia Medical Partnership. Dr. Fekete is a member of Alpha Omega Alpha Honor Medical Society and a Fellow of the College of American Pathologists.
 
Course information: This course provides a thorough review of cellular elements encountered in normal bone marrow. It also provides guidelines for preparation of smears, including an instructional video, and evaluation and reporting of bone marrows.

Bone Marrow Aspiration: Normal Hematopoiesis and Basic Interpretive Procedures Keywords

These are the most common topics and keywords covered in Bone Marrow Aspiration: Normal Hematopoiesis and Basic Interpretive Procedures:

phagocytic myeloblasts nucleoli lineage immune bone marrows band neutrophil fixative clinical body rack basophils cytoplasm megakaryocytes centrally-placed histiocytes nuclear normoblasts condensation tumor laboratory anticoagulants readable monocytes erythroid manageable blast-like anticoagulated immature polychromatophilic hematopathologist heparin t-prep basophilia platelet syringe counterpart pronormoblast hemophagocytosis cellular clinicians oncology knotted rbcs assays cytoplasmic eosinophils circulating dozen nucleus specimens binucleate aspiration myelocyte indistinguishable basophilic matured differentials usable methanol-based mounted thrombocytopenia disorders shade blood coverslips granulocyte dexterity preservative-free bone marrow transferred osteoblasts numbers professionals gritty neutrophils needle sand erythroids identification condense myeloid morphology micro-pipette golgi cytometry nucleolus vacuoles heart wright cytopenias intimidating grains monolayer infrequently normoblast macrophage sharp preference precursor hematology appears monoblasts segmented neutrophils anemia velvety-look compact sectioned hemoglobin touch-preparation biopsies cytogenetics cells macrophages nuclei coarser pathology megakaryocyte technologists leukemic maturational promyelocyte settings protocols granules pores toxic samples wright-giemsa clusters hematogones workspace perinuclear lymph plasma cells rinsed promyelocytes lacy bony peripheral blood smear myeloblast segmented neutrophil leukemia aspirates myelocytes lymphoid granulated monocyte biopsy differential decalcified roll lymphoblasts platelets iron lighten preparations coverslip pathologists evaluation histiocyte lineages t-cells clumpy diagnosis lymphocytes nrbcs red blood cells pipette b-lymphocytes noticeable hypocellular artifact antigens chromatin spicules clinician shrink pediatric stromal non-nucleated leukemias buffer diagnostic lymphocyte hematopathologists aspirate anemias cellularity methods transformation midnight-blue osteoclasts syringes marrow lymphoblast precursors indent erythrocyte metamyelocyte



band 2 sml


cellularity normal sml


plasma 2 sml


2jmml13 sml with arrows


bm baso + poly sml with arrows 2


young megka 1 sml with arrow


tprep sml LC updated


progranulocyte sml updated 2