| Discussion A phlebotomist should never use an arm with restricted usage for the venipuncture. Even if no sign is posted, the patient may tell you not to use a particular arm for various reasons, i.e. previous mastectomy, history of phlebitis, active AV fistula, etc. Do not draw blood above an IV line. If blood is taken from a vein above an IV line it might be diluted by the IV fluid, which could cause incorrect test results. In this case, Bobby should choose a vein on the dorsum of Mrs. Grayson’s hand, below the IV. A butterfly needle would facilitate drawing blood from these small hand veins.Relevant topics:Alternate sites, Sites to avoid, Signs, Arms to avoid
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| Case Bobby Jones, a phlebotomist at Georgetown Hospital, entered the room of Mrs. Mary Grayson with a physician's order to draw some blood work. After properly greeting Mrs. Grayson, identifying himself and checking her armband, Bobby prepared for the venipuncture. He suddenly notice a sign posted above the bed that read: “Restricted left arm usage. Previous mastectomy - Do no use left arm for venipuncture.” Bobby set up his equipment to use her right arm and noticed an IV line in Mrs. Grayson’s right arm positioned in a vein slightly above her wrist on the dorsum (top) of her forearm. | View Page |
| Which site should Bobby choose for the venipuncture? | View Page |
| Case Julie Smith, a newly certified phlebotomist at Northlake Hospital, entered a patient’s room on the third floor for a routine blood draw. The patient was an elderly woman who had very small fragile veins. Julie therefore decided to use a safety butterfly needle attached to a Vacutainer tube in order to draw the blood. When Julie was finished with the venipuncture, she detached the butterfly needle from the Vacutainer, and approached the Biohazard needle disposal box. She noticed that the disposal box was full , but decided to try to fit the butterfly into the box anyway. Holding the butterfly by the tubing, she tried to push the butterfly into the box. The needle suddenly recoiled and stuck Julie’s finger. Julie left the patient’s room in a panic and headed back to the lab to report the needle stick injury. | View Page |
| Discussion Insufficient blood volume may cause erroneous test results, and specimen rejection. When blood flow stops, it can mean several things:The bevel of the needle may be pressed against the wall of the blood vessel. If this is the case, moving the needle slightly may cause blood to begin flowing again.The vein may have collapsed due to the vacuum of the tube. If moving the needle slightly does not re-establish blood flow, you will have to recollect the patient.The needle may have gone all the way through the vein. Pulling the needle back slightly may cause blood to resume flowing.
The tube you are using may have insufficient vacuum. Try another tube. Never vigorously probe the patient’s arm with a needle. At the first sign of discomfort the needle should be withdrawn. The patient may then be redrawn be yourself or another phlebotomist.Relevant topics: Insufficient volume, Partial collection tubes, What if no blood flows | View Page |
| Tourniquets Tourniquets wrap around the arm to increase venous pressure, and fill the veins, so that they are easier to see, feel, and puncture.
They can be made of latex, Velcro, or other material.
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| Multiple draw needles with built-in safety features. You will be required to use multiple draw needles with built-in Safety features.
One example is the Puncture-Guard™ (BioPlexus) needle, which uses an internal blunt needle (detail above) that is activated with forward pressure on the final blood tube prior to withdrawal of the needle from the vein.
Refer to your institution’s and the manufacturer’s procedure manuals before using these devices.
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| Choosing a site The median cubital vein is most commonly used. It is the middle of the bend of the arm (antecubital region).
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| Patient clenches fist After applying the tourniquet, you may ask the patient to make a fist to further distend the arm veins. | View Page |
| Applying the tourniquet Tie the tourniquet just above the elbow.The tourniquet should be tight enough to stop venous blood flow in the superficial arm veins. | View Page |
| Choosing a site continued If the median cubital vein is not accessible, try the: Cephalic vein, or the Basilic vein. | View Page |
| Alternate Sites If no veins are accessible on the arm, you may try the veins on the back of the hand.Use a much smaller needle for these hand veins. | View Page |
| Hold vein in place You may now hold the patients arm below the venipuncture site.Pull the skin below the vein to anchor it and keep it from rolling. | View Page |
| Insert needle With the needle bevel up, insert the needle into the vein. | View Page |
| Blood won't flow If you do not see blood flow, the tip of the needle:May not yet be within the vein.May have already passed through the vein.May have missed the vein entirely.May be pushed up against the inside wall of the vein. | View Page |
| Butterfly needle - Butterfly needle collections Butterfly needles (also known as a winged infusion set), are available in smaller gauges, and are used to draw venous blood from children, and adults with difficult veins.
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| Butterfly needle - Angel WingTM safety butterfly The Angel Wing™ (Monoject), is activated by sliding a stainless steel safety shield over the needle after withdrawal from the vein.The Angel Wing™ (Monoject), is activated by sliding a stainless steel safety shield over the needle after withdrawal from the vein. | View Page |
| Syringe - Syringe blood collections Syringes may be used to collect blood from patients having small or delicate veins that might be collapsed by the vacuum of the evacuated tube system.Syringes may also be used to collect blood culture specimens. | View Page |
| Heelstick - Pediatric collection procedures: Introduction Veins of small children and infants are too small for venipuncture;Safety Lancets are used to puncture the skin and collect capillary blood.Butterfly needles may be used to collect venous blood in older children.
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| Bleeding time Select a site on the inside of the forearm with no visible veins, scars or bruises, about 5 cm below the bend of the elbowNicking a vein will invalidate the result. | View Page |
| Feel the vein If you need to feel for a vein again, you must first prep your gloved finger tip with iodine to prevent contamination. | View Page |
| Hematosis A hematoma is a blood clot which forms within the body. It is caused by leakage of blood into the tissues from an injured vein . It will resolve spontaneously.Hematomas are caused by excessive needle trauma to a vein, for example, by a needle which passed entirely through a vein and came out the other side.Apply compression to help stabilize a hematoma.
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| Blood should not be drawn from arms with IVs Blood drawn from veins with intravenous lines (IVs) may be diluted by the IV fluids. Arms containing IVs should therefore not be used to draw blood specimens.
If an arm with an IV line in place must be used for venipuncture, be sure to choose a site below the location of the IV, so that the specimen will not be diluted with IV fluids. | View Page |
| What if no blood flows when the needle is in place? The needle may not be in a vein. Try slightly manipulating the needle. If no blood flows, withdraw the needle and repeat the venipuncture. Never probe the patient’s arm with the needle. The bevel of the needle may be compressed against the inside of the vein wall. Slightly manipulating the needle should result in blood flow.
The needle may have passed entirely through the vein. Pull it back slightly, and blood should flow. | View Page |
| Insufficient volume Insufficient blood volume (short draws) within a collection tube containing anticoagulant will result in an incorrect ratio of blood to anticoagulant, and yield incorrect test results.Short draws can be caused by: A vein collapsing during phlebotomy.The needle coming out of the vein before the collection tube is full.Loss of collection tube vacuum before the tube is full. (Always keep extra tubes on hand.) | View Page |
| What is phlebotomy? Phlebotomy, also known as venipuncture, means collecting blood from veins.Phlebotomists, by definition, collect venous blood, but perform a variety of other important medical tasks as well. | View Page |
| Circulation: venous portion Deoxygenated (venous) blood flows from tiny capillary blood vessels within the tissues via progressively larger veins to the right side of the heart.Blood is routinely drawn from veins, but may also be drawn from arteries, or capillaries.
Illustration this screen from LifeArt Collection 2000, with permission. © Lippincott Williams & Wilkins.
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| Circulation: capillary portion In the capillaries, oxygen and nutrients diffuse from the blood cells into the tissues.
The deoxygenated blood then returns to the veins, completing the circulatory pathway.
Illustration this screen from LifeArt Collection 2000, with permission. © Lippincott Williams & Wilkins.
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| Anatomy & physiology: essential to phlebotomy Since phlebotomy involves puncture of the skin (integumentary system) and veins,
(A component of the cardiovascular system), a basic knowledge of the anatomy and physiology of these systems is essential.
Knowledge of blood and its components is also important.
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