Chemical Screening of Urine by Reagent Strip (Online Course)
Lillian Mundt, MHS, MT(ASCP), SH
This course discusses the chemical tests that are an integral part of a urinalysis. Proper specimen handling and technqiue are discussed for key tests, including pH, specific gravity, protein, glucose, ketones, bilirubin, blood, nitrites, urobilinogen, and leukocyte esterase.
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Continuing Education Credits
- P.A.C.E.® Contact Hours: 1 hour(s)
- Florida Board of Clinical Laboratory Science CE - General (Clinical Chemistry/UA/Toxicology): 1 hour(s)
Objectives
- Define the optimal storage time and temperature for urine specimens that will be tested using the reagent strip method.
- Describe the proper storage and procedure for the use of reagent strips.
- For routine screening tests included on the reagent strip (pH, specific gravity, protein, glucose, ketones, bilirubin, blood, nitrites, urobilinogen, leukocyte esterase) describe: chemical reaction used for its determination, quality control measures, interpretation of results, clinical significance.
- Describe the proper technique to use for urine reagent strip testing to ensure accurate results.
- Identify conditions and/or substances that may interfere with urine reagent strip testing.
- Identify tests that may be used to confirm reagent strip test results.
- Explain the principle of the machines used for reading reagent test strips.
Course Outline
Click on a link in the outline to view a sample page from this course.
- Introduction to Reagent Strips
- Testing pH
- Basis of the Urine pH Test
- pH Value
- Acid and alkaline urine pH
- Urine Specimen
- The indicator(s) used in the pH test region is/are: (Choose ALL of the correct answers)
- The pH range that can be determined on the urine reagent strip is:
- A urine specimen was collected at 6:00 A.M. and remained at room temperature until it was received in the laboratory at 3:30 P.M. How may the pH of th...
- Match the following factors with the expected pH:
- Testing for Protein
- Protein Error of Indicators
- False Positive Protein Results
- Confirmatory Testing for Protein
- Clinical Significance
- Clinical Significance cont'd
- Clinical Significance cont'd
- Clinical Significance cont'd
- Follow-up Testing of Urine Dipstick Protein Results
- The ability of proteins to alter the color of tetrabromphenol blue without altering the pH is an app
- Tetrabromphenol blue is buffered at pH of:
- Proteinuria related to kidney impairment may be due to: (Choose ALL of the correct answers)
- Proteinuria that may signal impending damage is seen in: (Choose ALL of the correct answers)
- Examples of conditions resulting in benign proteinuria include: (Choose ALL of the correct answers)
- Testing for Glucose
- Glucose Test
- False Positive Results
- False Negative Results
- Timing
- Test for Reducing Substances Other than Glucose
- Clinical Significance
- Clinical Significance cont'd
- Which of the following substances can result in a false negative test result? (Choose ALL of the cor
- A copper reduction method (e.g. Clinitest® or Benedict's) is performed on pediatric specimens in order to check for the presence of:
- Which of the following conditions produce glycosuria? (Choose ALL of the correct answers)
- Testing for Ketones
- Three Kinds of Ketones
- Testing for Ketone Bodies
- Ketone Bodies
- False Positive Ketone Results
- False Negative Results
- Clinical Significance of Positive Urine Ketone Result
- The ketone component that is measured by the nitroprusside reaction is:
- The reagents used in the nitroprusside reaction are: (Choose ALL of the correct answers)
- Which of the following substances can cause a false positive result for ketones? (Choose ALL of the
- Significantly increased levels of ketones are detected in the urine with which of the following conditions? (Choose ALL of the correct answers)
- Testing for Bilirubin
- Bilirubin Characterization
- Test for Bilirubin
- False Positive Results
- False Negative Results
- Confirmatory Testing
- Clinical Significance
- Diazotized 2, 4, dichloroaniline reacts with bilirubin in an ___________ medium.
- Which of the following may cause a false positive bilirubin result on a urine reagent strip?
- Which of the following may cause false negative bilirubin results on a urine reagent strip? (Choose ALL of the correct answers)
- When a patient has a bile duct obstruction, the bilirubin test portion of the reagent strip is:
- Testing for Blood
- Hematuria
- The Test for Blood
- False Positive Results
- False Negative Results
- Clinical Significance
- A urine sample is cloudy pink in appearance. The microscopic examination reveals the presence of in
- Which of the following substances cause a false positive result for blood on the urine reagent strip? (Choose ALL of the correct answers)
- Which of the following substances may cause a false negative result for blood on the urine reagent strip? (Choose ALL of the correct answers)
- Match the following:
- Testing for Nitrites
- Nitrite Test
- Bladder Infections
- Test Sensitivity
- False Positive Results
- False Negative Results
- Clinical Significance
- Nitrates in urine are reduce to nitrites by:
- Match the following:
- The nitrite portion of the test strip can be used to: (Choose ALL of the correct answers)
- Testing for Urobilinogen
- Urobilinogen
- The Test for Urobilinogen
- False Positive Urobilinogen Results
- False Negative Urobilinogen Result
- Confirmatory Test
- Clinical Significance
- The color change in the Ehrlich Aldehyde reaction is:
- Which of the following substances cause a false-positive reaction on the urobilinogen portion of the
- Which of the following cause false negative reactions when testing for urobilinogen with the reagent strips? (Choose ALL of the correct answers)
- A test that can be used as a confirmatory test for urobilinogen is:
- Urobilinogen is excreted in the urine in increased amounts in: (Choose ALL of the correct answers)
- Testing for Leukocyte Esterase
- Granulocytic white blood cells
- Leukocyte Esterase Dipstick Test
- False Positive Leukocyte Esterase Test
- False Negative
- Clinical Significance
- A positive leukocyte esterase test indicates the presence in a urine specimen of which of the following?
- False-negative results on reagent strips for leukocytes may occur when the specimen contains: (Choos
- To screen for urinary tract infections leukocyte esterase should be coupled with: (Choose ALL of the correct answers)
- Testing for Specific Gravity
- Measuring Specific Gravity
- Advantages and Limitations of the Reagent Strip Method for Specific Gravity
- Clinical Significance
- How does ion concentration in the urine relate to specific gravity?
- A visually-read specific gravity on a urine specimen with an alkaline pH should be adjusted by:
- Automation of Reagent Strip Analysis
- Semi-Automated Instruments
- Quality Control
- Semi-automated and automated reagent strip readers:
- Urine reagent strips readers use any manufacturers dipsticks.
- When an automated or semi-automated method is used to read urine reagent strips, quality control testing must be performed at least:
Additional Information
Level of instruction: Basic Intended audience: Clinical laboratory science students, and medical technologists and technicians seeking review, cross training, or continuing education opportunities. It is also appropriate for other medical personnel who perform urine dipstick testing at the point of care. Author information: Lillian Mundt, MHS, MT(ASCP)SH holds a full time appointment within the College of Health Professions, in the Department of Clinical Laboratory Sciences at Rosalind Franklin University, where she is responsible for various courses in the undergraduate and graduate programs. Her primary discipline is Hematology and Body Fluid Analysis. Additionally, Lillian is responsible for the development of on-line courses and continuing education activities for the department, as well as acting as the department's chair. About the Course: This course is part of a series of courses adapted for the web by MediaLab Inc. under license from Educational Materials for Health Professionals Inc. Dayton OH, 45420. Copyright EMHP.
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