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Underdecolorized Information and Courses from MediaLab, Inc.

These are the MediaLab courses that cover Underdecolorized and links to relevant pages within the course.

Learn more about laboratory continuing education for medical technologists to earn CE credit for AMT, ASCP, NCA, and state license renewal and recertification. Or get information about laboratory safety and compliance courses that deliver cost-effective OSHA safety training and continuing education to your laboratory's employees.

Laboratories Individuals

Reading Gram Stained Direct Smears
Regarding the smear shown in this illustration, which of the following is true?View Page
Regarding the smear shown in this illustration, which of the following is true? (Choose ALL of the correct answers)View Page
Regarding the smear shown in this illustration, which of the following is true? (Choose ALL of the correct answers)View Page
Evaluation of Controls

If stains and technique are adequate, S. aureus should be gram positive (blue) and E. coli should be gram negative (pink). If control slides do not react appropriately, reliable results cannot be assured for the specimen smears. Check stains and technique and prepare more control smears until proper results are achieved, then remake and stain the new direct smears. If it is impossible to prepare a new smear, the poorly stained smear may still be salvaged. Remove immersion oil from the smear using xylol. Use appropriate procedures and personal protective equipment when using xylol, since it is hazardous chemical. If the smear is underdecolorized, repeat the decolorization and counterstain steps. If the smear is overdecolorized, the slide should be stained again.

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