Transcriptase Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Transcriptase and links to relevant pages within the course.
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| Amplified Nucleic Acid Testing Principle These methodologies use principles that amplify or multiply the target of interest, usually incorporating an enzyme to produce millions or billions of copies in a relatively short time.Some enzymes used in amplification include: DNA ligase DNA polymerase RNA polymerase Reverse transcriptase Alkaline phosphatase Cleavase Note: the steps in amplified testing will vary depending on the target amplified, requirement for thermal cycling and detection techniques. | View Page |
| Amplified Nucleic Acid Tests Amplification Method Amplifies Use of Thermal Cycling (Thermocycling) Polymerase Chain Reaction (PCR) Target amplification using DNA polymerase Yes Ligase Chain Reaction (LCR) Target amplification using DNA ligase Yes Transcription- based or Transcription-mediated amplification(TMA) Target amplification using reverse transcriptase and RNA polymerase No Strand Displacement (SDA) Target amplification using DNA polymerase that continuously displaces strands of DNA containing the target sequence No Branched DNA (bDNA) Signal amplification using alkaline phosphatase No Loop Mediated (LAMP) Target amplification of multiple DNA sequences in a loop pattern using DNA polymerase No Nucleic acid sequence based (NASBA) Target amplification using 3 enzymes No Q-beta Replicase Probe amplification- The concentration of an RNA probe increases if the target is present No | View Page |
| Match the following tests to their appropriate principle: | View Page |
| Retrovirus The Human Immunodeficiency Virus type-1 (HIV) belongs to the Family Retroviridae.In HIV, RNA is the template for the synthesis of DNA. This differs from most cellular biochemistry in which DNA is used as the template for the synthesis of RNA.The enzyme that transcribes the RNA for the synthesis of DNA is called reverse transcriptase.Because of the enzyme's activity, HIV is known as a retrovirus - retro implying reverse. | View Page |
| The stage of viral replication where the envelope is being acquired and the HIV is leaving the host cell is known as: | View Page |
| What is the source of the HIV envelope? | View Page |
| Which enzyme is responsible for joining the HIV DNA to the host cell's DNA? | View Page |
| Spread of Infection (1) The proviral DNA provides genetic coding that instructs cellular enzymes to construct new HIV genomes, capsid proteins, and reverse transcriptase molecules.All of these are assembled near the edge of the host cell. | View Page |
| HIV is a Retrovirus In most cellular biochemistry, DNA is used as the template for the synthesis of RNA.In HIV RNA is the template for the synthesis of DNA. That is why the enzyme is called reverse transcriptase.Because of the enzyme's activity, HIV is known as a retrovirus - retro implying reverse. | View Page |
| DNA Replication from RNA Once the capsid and p24 and p17 have been stripped away, an enzyme complex known as reverse transcriptase is released.One of the enzymes in this complex is DNA polymerase. It synthesizes a single-stranded DNA copy using one of the HIV-RNA molecules as a template.Another enzyme in this complex, called ribonuclease, then destroys the original RNA molecules while the DNA polymerase synthesizes another single-stranded DNA molecule, this time using the first DNA copy as the template.The result is a double-stranded DNA molecule. | View Page |