Thermocycling Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Thermocycling and links to relevant pages within the course.
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| Amplification As seen in the preceding table of amplified nucleic acid test methodologies, any of the following can be amplified: Target (most common) Probe Signal When employing an amplification procedure, each methodology can differ based on: Amount of target Amplification type Enzyme requirements Thermal cycling (thermocycling) requirements Detection methodology | View Page |
| Amplified Nucleic Acid Tests Amplification Method Amplifies Use of Thermal Cycling (Thermocycling) Polymerase Chain Reaction (PCR) Target amplification using DNA polymerase Yes Ligase Chain Reaction (LCR) Target amplification using DNA ligase Yes Transcription- based or Transcription-mediated amplification(TMA) Target amplification using reverse transcriptase and RNA polymerase No Strand Displacement (SDA) Target amplification using DNA polymerase that continuously displaces strands of DNA containing the target sequence No Branched DNA (bDNA) Signal amplification using alkaline phosphatase No Loop Mediated (LAMP) Target amplification of multiple DNA sequences in a loop pattern using DNA polymerase No Nucleic acid sequence based (NASBA) Target amplification using 3 enzymes No Q-beta Replicase Probe amplification- The concentration of an RNA probe increases if the target is present No | View Page |
| Thermal Cycling Thermal cycling (thermocycling) uses specific instrumentation to cause variations in temperature during an amplification procedure in order to produce copies of the target of interest (or amplify the target of interest) so that detection can take place.The temperature variations increase to unfold the DNA and then decrease to allow for reconnection or reannealing. | View Page |