Sulfosalicylic Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Sulfosalicylic and links to relevant pages within the course.
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| Sulfosalicylic acid can be used to confirm the result of which of the following tests included on a urine reagent strip? | View Page |
| Confirmatory Testing for Protein Semiquantitative tests are used in some laboratories to confirm the presence of protein in the specimen when the result is positive on the urine dipstick. Tests that are used for confirmation include: sulfosalicylic acid (SSA); heat and acetic acid; nitric acid ring test; and Roberts' Ring Test. Any one of these procedures may be used for confirmation of the presence of protein. A protein dipstick result that is greater than a trace may be an indication of proteinuria. | View Page |
| The ability of proteins to alter the color of tetrabromphenol blue without altering the pH is an application of: | View Page |
| Which of the following tests confirms the presence of Bence-Jones proteinuria: | View Page |
| Which of the following methods is not a quantitative method for the determination of
albumin: | View Page |
| Which of the following methods would be used to confirm the presence of Bence-Jones
protein in the urine: | View Page |
| Which of the following would be the most appropriate method to confirm a positive
protein from a urine dipstick: | View Page |
| Screening and Secondary Tests for Protein A routine reagent strip protein method, based on the principle of "protein error of indicators," produces a visible colorimetric reaction that is capable of detecting most instances of proteinuria.Traditionally, laboratories have used sulfosalicylic acid (SSA) to confirm all positive protein reagent strip results, but this practice is not as common in today's laboratories. SSA is a precipitation method that reacts with all forms of protein. However, any substance that is precipitated by acid will produce false-positive SSA results. This includes radiographic dyes, cephalosporins, penicillins, and sulfonamides. SSA may be used as a secondary protein detection method if the urine is highly alkaline (pH of 9.0 or greater) which would overwhelm the buffering capacity of the reagent on the protein reagent stick. SSA may also be used as an alternative protein detection method if the urine is highly colored so that the colorimetric reaction is masked on the reagent strip. | View Page |
| Sulfosalicylic Acid Test (Exton's Modification) There are several acids which can be used to precipitate proteins - sulfosalicylic, trichloroacetic, nitric, and acetic acids. Sulfosalicylic acid (SSA) is the most frequently used acid test because it does not require the use of heat. Exton’s reagent is 5% sulfosalicylic acid in a solution of sodium sulfate. Exton (1925) found that adding sodium sulfate to the SSA causes a more uniform precipitate to be formed. To perform the SSA procedure mix equal parts of patient urine and the reagent. Rate the amount of turbidity according to the following scale: | View Page |