Staphylococci Information and Courses from MediaLab, Inc.
These are the MediaLab courses that cover Staphylococci and links to relevant pages within the course.
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| Which of the following organisms will give a positive coagulase test: | View Page |
| Bacitracin susceptibility is useful for which two of the following: | View Page |
| Which one of the following organisms is sensitive to bacitracin: | View Page |
| A gram stain of the serous exudate is shown in the photograph. The appropriate report would read: | View Page |
| MRSA Screen Perhaps the most efficient means for detecting methicillin-resistant staphylococci in clinical laboratories is the use of the agar dilution screening test.Illustrated in the photograph is a Mueller-Hinton agar plate containing 6ug/ml of oxicillin, previously inoculated with a strain of Staphylococcus aureus. Oxacillin is used as a marker for methicillin resistance because it is more stable in the agar medium. Growth on this screening medium is presumptive for methicillin resistance.Thus, in the presence of growth, as shown here, a follow-up MIC test must be performed to determine the exact level of resistance. | View Page |
| MRSA Disk Test The disk diffusion test can also be used in the detection of methicillin-resistant Staphylococcus aureus.Illustrated in the photograph is the surface of a Mueller-Hinton agar plate previously inoculated with a strain of S. aureus suspected of being methicillin-resistant. Although the zone of inhibition is at the borderline for resistance (18 mm); the presence of small colonies within the zone of inhibition (yellow arrows) indicates the presence of heteroresistant strains.The interpretation here, therefore, is "methicillin-resistant" staphylococci, even though the zone diameter appears to be adequate. The detection of the heteroresistant strains indicates that MIC studies are required. | View Page |
| Beta hemolytic colonies grew from the blood culture bottle after 18 hours incubation (see photograph). The following tests would be helpful in making a preliminary identification: | View Page |
| Gram positive cocci (GPC) Gram positive cocci(GPC) can be seen in clumps and chains in this picture.
Gram positive cocci(GPC) in chains may represent streptococci. Gram positive cocci in clumps may represent staphylococci. | View Page |