Cell counting techniques

Calculation of CSF Cell Count

In general, use the following equation to calculate CSF cell count:

  • (total cells counted x dilution) / (number of squares counted x volume of 1 square) = cells per microliter.
For an undiluted specimen in which 10 squares are counted:

  • (total cells counted x 1) / (10 squares counted x 0.1 mm3 per square) = cells per microliter.
Therefore, in this example:
  • (total cells counted) / (1 mm3) = cells per microliter.

1 mm3 is equal to 1 microliter.

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