Wet mount assessment of sperm motility
A wet mount evaluation of motility involves examining at least five fields and/or a minimum of 200 sperm using the high power objective. For very low counts, increased numbers of fields may have to be examined and the total number of sperm may be less than 100. Two different slides are made and the percent motility is determined using different aliquots of the specimen. Results should agree within 10% of each other or a third aliquot is used and the average of the three is taken as the result.