Some professionals believe that sperm counts done by hemacytometer are not accurate because of the need to dilute the viscous semen prior to counting. There are several other counting methods available to assess sperm concentration.

The advantages of the following methods are:

• the specimen does not have to be diluted
• motile and non-motile sperm can both be counted avoiding the need for wet mount evaluation of motile cells.
  Note that counting moving sperm can be difficult and takes significant practice to avoid error.
  
  

For each of these methods accurate counts are best obtained when at least 100 sperm per replicate are counted.

• Makler (Zygotek Systems, Inc.). An undiluted sample is placed on the chamber and covered with the coverglass. Ten squares on the grid contain 0.000001ml.
• CellVu (Millennium Sciences, Inc). Two sides of a special slide are loaded with a drop of undiluted semen. Coverslips with special grids are placed on top of the sperm according to manufacturer's directions. Sperm on both sides are counted.
• MicroCell (Conception Technologies) has two chambers on a single, disposable slide. A special eyepiece with a grid is needed for counting.