The test for urobilnogen is based on the Ehrlich Aldehyde Reaction. P-dimethylaminobenzaldehyde in an acid medium with a color enhancer reacts with urobilinogen to form a pink-red color.
The urine chemical reagent strip reactivity increases with increasing temperature. The optimum temperature for testing is 22° - 26°C.
A freshly voided sample is always best for ensuring optimal results.