The Clinitest® method can detect reducing substances in the urine up to 2 g/dL. When the amount of sugar is over 2 g/dL (often expressed as 2%), a “pass through” phenomenon occurs. Pass through appears as rapid color changes through green, tan, and orange, and then a reversion in color back to the brownish color.
This reversion in color indicates levels of reducing substances greater than 2 g/dL. Even a fleeting orange color should be recorded as “greater than or equal to 2 g/dL.”
It is vital that you watch the boiling and color changes throughout the entire reaction so that a "pass through" is not missed.