SSA Procedural Notes and Test Limitations

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SSA Procedural Notes and Test Limitations

  • When testing for protein using the SSA precipitation method, a urine sample that is not clear should be centrifuged and the supernatant used for testing.
  • Daylight or fluorescent light is recommended when interpreting results.
  • Quality control checks should be performed by testing known negative and positive specimens or controls.
False-positive results can occur when the urine specimen:
  • Contains x-ray contrast media
  • Contains a high concentration of certain antibiotics
  • Is turbid
False-negative results can occur when the urine specimen:
  • Is a highly buffered alkaline urine (may need to acidify the urine to a pH of 7.0 or lower before performing the SSA test).
  • Is very dilute
  • Is turbid