The pH of the urine must be approximately 7 or less in order to avoid false negatives with this test. If the pH exceeds 7, add 33% acetic acid dropwise to acidify the specimen to a pH of 7.

If the specimen is cloudy, it should be centrifuged before SSA reagent is added.

Daylight or fluorescent light is recommended when interpreting results.

Quality control checks should be performed by testing known negative and positive specimens or controls.