This is an example of a mix of homogeneous and centromere ANA patterns.
In this sample notice the smooth homogeneous pattern of the interphase cells and smooth staining of the chromosomal area of the mitotics (a).
Also notice the discrete speckling of the centromere pattern present in the nucleus of the interphase cells as well as in the chromosomal area of the metaphase mitotic cells (b).
In cases of mixed homogeneous and centromere ANAs, follow-up testing for anti-dsDNA is necessary.
When mixed patterns are titered, the endpoint for each pattern is reported.
This pattern is reported as ANA positive, Homogeneous and Centromere; titering is necessary.
Homogeneous and Centromere:
Interphase cells
- Smooth staining of entire nucleus
- Usually 46 discrete speckled
Metaphase mitotic cells
- Smooth staining of the chromosomal region
- Discrete speckles align in the chromosomal region of the mitotics
- Region outside of the chromosome area will stain with variable intensity
