This is an example of a Midbody ANA pattern.
In this pattern there is no staining in the early interphase cells (a). By late interphase fine speckling is present (b). In metaphase the speckling is located in chromosomal area and is very similar to centromere staining (c). As the cells progress into anaphase and telophase staining is seen in the intercellular bridge (d).
No follow-up testing is necessary.
This pattern is reported as ANA positive, Midbody; titering is optional.
Midbody:
Interphase cells
• Speckled staining appears in late interphase
Prophase cells
• Discrete speckling resembles Centromere
Metaphase mitotic cells
• Discrete speckling in chromosomal region resembles Centromere
Anaphase, Telophase and Cytokenisis cells
• As cells separate it becomes apparent the staining is in the cleavage furrow between the dividing cells
