NSp II or CENP-F

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NSp II or CENP-F

This is an example of the ANA pattern seen with antibodies to CENP-F or NSp II.

This pattern is characterized by the absence of staining in the interphase cells (a). In late interphase speckling begins to appear (b). The metaphase mitotics demonstrate staining similar to centromere pattern but the speckling is often in two rows (c).

No follow-up testing is available. These antibodies are seen inpatients with various cancers.

This pattern is reported as ANA positive, NSp II or CENP-F; titering is optional.

CENP-F or NSp II:

Interphase cells

  • Speckled staining appears in late interphase

Prophase cells

  • Look like Centromere

Metaphase mitotic cells

  • Look somewhat like Centromere