This is an example of a Multiple Nuclear Dots (MND) ANA pattern.
This pattern is characterized by several (15-30) discrete speckles, or dots, in the nucleus of the interphase cells (a). There is no staining in the nucleoli and the chromosome area of the metaphase mitotics is negative (b). This pattern is distinguished from centromere by having fewer speckles and negative mitotics.
Follow-up testing for antibodies to Sp-100 is available. These antibodies are seen inpatients with primary biliary cirrhosis.
This pattern is reported as ANA positive; unusual or atypicial speckled; titering is optional.
NSp I or MND:
Interphase cells
- 15-30 discrete speckles
- Evenly distributed in the nucleus
Metaphase mitotic cells
- No staining in chromosomal region
- Region outside of the chromosome area will stain with variable intensity
