Homogeneous | Double stranded DNA (dsDNA) | Characteristic of SLE, lower levels in other rheumatic diseases | IFA or CZ using Crithidia luciliae, RIA, ELISA, Addressable Laser Bead Assay (ALBIA) | |
Nucleosome or Chromatin | SLE, Drug-induced LE | ELISA | |
Histone | Drug-induce LE, SLE | ELISA, ALBIA | |
Unusual Homogeneous | Nuclear Membrane | Lupoid hepatitis | ELISA for gp-210 | |
Speckled | Sm (Smith) | Marker antibody for SLE | Immunodiffusion (ID), ELISA, ALBIA | |
U1-RNP | High levels in MCTD and SLE, low levels in other rheumatic diseases | ID, ELISA, ALBIA | |
Speckled (and/or SSA pattern if using HEp-2000®) Can also be ANA negative | SS-A/Ro | High prevalence in Sjögren syndrome sicca complex, lower prevalence in other rheumatic diseases | With HEp-2000 characteristic ANA pattern is confirmatory, others confirm with ID, ELISA, ALBIA | |
Fine speckled or ANA negative | Ro52 | Sjögren syndrome, myositis, Neonatal Lupus | ELISA, ALBIA | |
Fine speckled (sometimes with nucleolar staining as well) | SS-B/La | High prevalence in Sjögren syndrome sicca complex, lower prevalence in other rheumatic diseases | ID, ELISA, ALBIA | |
Fine speckled, Homogeneous, Nucleolar | Scl-70 | Marker antibody for Scleroderma | ID, ELISA, ALBIA | |
Cell Cycle Dependent Speckled | PCNA | Marker antibody for SLE | ID, ELISA, ALBIA | |
Coarse Speckled | Nuclear Matrix | Seen in some patients with evolving connective tissue disease | NONE | |
3-20 dots | NSp I, sp-100, MND, PBC 95 | Associated with Primary Biliary Cirrhosis | ELISA, ALBIA | |
Cell Cycle Dependent Speckled with speckling in metaphase mitotics | NSp II, CENP F | Some association with malignancies | NONE | |
Staining in cleavage furrow between dividing cells | Midbody | Unknown | Confirm by staining pattern | |
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Centromere | CENP A, CENP B, CENP C | Seen in 57-82% of patients with limited form (CREST) of scleroderma and Raynaud phenomenon | Confirmed by staining pattern ELISA, ALBIA | |
Nucleolar | Fibrillarin (Clumpy nucleolar) | Scleroderma | ELISA, ALBIA | |
RNA polymerase I, NOR-90, others? (Speckled nucleolar) | Scleroderma and other connective tissue diseases | ELISA, ALBIA | |
PM-1 (PM/Scl), others?(Smooth nucleolar) | Polymyositis/Scleroderma overlap | ELISA, ALBIA | |