Next the number of RBCs, WBCs, epithelial cells, parasites, and fat will be counted.
- Move to the center of the coverslip and examine 10 fields under high power (40X objective) brightfield. Use phase-contrast as needed.
- Determine the average number of each element found and record the findings as number per high power field (HPF).
- An abundance of any one element may be recorded as >100/HPF when 1/4 field is counted and the total field is estimated to be greater than 200/HPF.