Specimen Collection and Preparation

Steps in Preparing a Concentrated Urine Sediment

  1. Mix urine specimen well, and transfer 10-15 ml of urine to conical centrifuge tube.
  2. Centrifuge at 1500 rpm for 10 minutes.
  3. Decant supernatant, and resuspend sediment in 0.5-1.0ml of residual specimen.
  4. Place a drop of concentrated sediment on a glass slide and coverslip.

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