General Aspects of Gram Stained Culture Smears

Gram Stain

The Gram stain provides a fundamental differentiation between types of bacteria. Gram positive bacteria retain the primary stain and are deep violet in color. Gram negative organisms are those that decolorize during the staining process and, after counterstaining, are pink in color. Bacteria have two basic shapes:

  • cocci (round)
  • bacilli (rod-shape)

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