cTnT is also encoded by a separate gene and after translation, an 11-amino acid chain is added giving this protein its cardiac specificity. Its release characteristics and elevation periods are similar to cTnI. In some AMI patients, a second increase in analyte concentration occurs. It is believed that there are two pools of cTnT, cystolic and structurally bound pools. The first elevation is from the cystolic pool and the second elevation is from the structurally bound pool released later in the necrotic process.