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Most blood collection tubes contain an additive that either accelerates clotting of the blood (clot activator) or prevents the blood from clotting (anticoagulant). A tube that contains a clot activator will produce a serum sample when the blood is separated by centrifugation and a tube that contains an anticoagulant will produce a plasma sample after centrifugation. Some tests require the use of serum, some require plasma, and other tests require anticoagulated whole blood.

The table below lists the most commonly used blood collection tubes.

Tube cap color	Additive	Function of Additive	Common laboratory tests
Light-blue	3.2% Sodium citrate	Prevents blood from clotting by binding calcium	Coagulation
Red or gold (mottled or "tiger" top used with some tubes is not shown)	Serum tube with or without clot activator or gel	Clot activator promotes blood clotting with glass or silica particles. Gel separates serum from cells.	Chemistry, serology, immunology
Green	Sodium or lithium heparin with or without gel	Prevents clotting by inhibiting thrombin and thromboplastin	Stat and routine chemistry
Lavender or pink	Potassium EDTA	Prevents clotting by binding calcium	Hematology and blood bank
Gray	Sodium fluoride, and sodium or potassium oxalate	Fluoride inhibits glycolysis, and oxalate prevents clotting by precipitating calcium.	Glucose (especially when testing will be delayed), blood alcohol, lactic acid