Post-Analytic Check of Data
This Case
With the panel done 2 weeks post-transfusion, 5 panel cells that were Jk(a+) reacted and 5 that were Jk(a-) did not. This yields a p value of 0.004, which is less than the standard of 0.05, and therefore is more than acceptable statistically.
In other words, an antibody other than anti-Jka would be expected to produce these panel results only 4 times in 1000 (which is pretty unlikely).
Th true p value is much lower because many more cells were tested than in the panel alone.
Concluding that the antibody is anti-Jka is further strengthened because the patient's red cells type as Jk(a-).
Learning points: The most important things to know about statistical tools such as p values are that they
- Relate to the probability of getting the observed results if the null hypothesis were true (the panel results were due to another antibody)
- Do not substitute for technical and clinical judgment.