Special Procedures: Hemoglobin Electrophoresis
Migration of Hemoglobin in Alkaline Electrophoresis
Of the hemoglobins normally present in an adult, Hb A migrates the fastest, followed by Hb F. Hb A2 moves only slightly from the point of origin near the cathode.
Abnormal hemoglobins show the following migration patterns:
Hb C migrates with Hb A2 near the cathode.
Hb S lies between Hb A2 and Hb F.
Hb H and Bart's hemoglobin are unstable and very fast moving, with Hb H being the faster of the two. They are located nearer the anode past Hb A .
Relative migrations of hemoglobin variants on alkaline electrophoresis can be seen below.
