Example of Dosage and/or Multiple Antibodies Influencing the Strength of Reactions

This version of the course is no longer available.
Need multiple seats for your university or lab? Get a quote
The page below is a sample from the LabCE course Antibody Detection and Identification (retired 12/6/2013). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Antibody Detection and Identification (retired 12/6/2013) (online CE course)
Example of Dosage and/or Multiple Antibodies Influencing the Strength of Reactions

Varying reaction strengths in the same phase could indicate antibody showing dosage, multiple antibodies, or both.
Jka and S are the antibodies that are present. Weaker reactions can be seen when either of the target antigens is present alone and/or in the heterozygous state on the cell.
4+ reaction in panel cell 1, 4 and 9: Both Jka and S are present
4+ reaction in panel cell 7 and 10: S present (homozygous)
3+ reaction in panel cell 6: Jka present (homozygous)
3+ reaction in panel cells 2 and 8: S present (heterozygous)
2+ reaction in panel cell 5: Jka present (heterozygous)